Akt-mediated phosphorylation of argonaute 2 downregulates cleavage and upregulates translational repression of MicroRNA targets.
Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, Novina CD, Orth AP
Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. PubMed Article
Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, Novina CD, Orth AP
Mol Cell. 2013 May 9;50(3):356-67. doi: 10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 59904 | pDEST26 Ago2 | Expresses 6xHis-Ago2 (N-termi tag) in mammalian cells |
| 59905 | pDEST26 Ago2 S387A | Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells |
| 59906 | pDEST26 Ago2 S387E | Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells |
| 59907 | pDEST26 Ago2 4A | Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cells |
| 60651 | pDEST26 Ago2 5A | Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells |
| 60652 | pcDNA 3.1 (+) FLAG-Ago2 S387A | Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells |
| 60653 | pcDNA 3.1 (+) FLAG-Ago2 S387E | Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells |
| 60654 | pHAGE GFP-Ago2 S387A | GFP-Ago2 S387A mutant lentiviral vector |
| 60655 | pHAGE GFP-Ago2 S387E | GFP-Ago2 S387E mutant lentiviral vector |
| 60656 | pGEX 4T-1 GST-Ago2 S387A | Expresses GST-Ago2 S387A mutant in mammalian cells |