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UbcH5B
(Plasmid #60443)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60443 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PETSUMO
  • Backbone manufacturer
    Novagen, modified
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 6400
  • Modifications to backbone
    SUMO tag was added to the PET3a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    UbcH5B
  • Species
    H. sapiens (human)
  • Entrez Gene
    UBE2D2 (a.k.a. E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B)
  • Promoter T7

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UbcH5B was a gift from Cynthia Wolberger (Addgene plasmid # 60443 ; http://n2t.net/addgene:60443 ; RRID:Addgene_60443)
  • For your References section:

    E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Wiener R, DiBello AT, Lombardi PM, Guzzo CM, Zhang X, Matunis MJ, Wolberger C. Nat Struct Mol Biol. 2013 Sep;20(9):1033-9. doi: 10.1038/nsmb.2655. Epub 2013 Aug 18. 10.1038/nsmb.2655 PubMed 23955022