PurposeConstitutive expression vector for Cre-recombinase containing ADE2 selection marker.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60932||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)1032
- Promoter mutant TEF1
/ Fusion Protein
- Driven from a mutant TEF1 promoter with 0.16x activity
- Cloning method Unknown
- 5′ sequencing primer catattttccgtcgatgttgaaatcc
- 3′ sequencing primer cgataccgtcgaggggcagagcc (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid contains I100V, K245R and F258L mutations in the ADE2 gene that do not impact selection or plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPL5606_TEF1*-Cre_ADE2 was a gift from Robert Piper (Addgene plasmid # 60932 ; http://n2t.net/addgene:60932 ; RRID:Addgene_60932)
For your References section:Puromycin and Methotrexate Resistance Cassettes and Optimized cre-recombinase Expression Plasmids for use in Yeast. MacDonald C, Piper RC. Yeast. 2015 Feb 16. doi: 10.1002/yea.3069. 10.1002/yea.3069 PubMed 25688547