pROSA26-Donor
(Plasmid #60952)

• Purpose: Porcine ROSA26 locus targeting
• Depositing Lab: Liangxue Lai
• Publication: Li et al Cell Res. 2014 Apr;24(4):501-4. doi: 10.1038/cr.2014.15. Epub 2014 Feb 7.

Backbone

• Vector backbone: pFlexibleDT
• Backbone size w/o insert (bp): 7200
• Vector type: Gene targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Porcine ROSA26 homologous ARM
• Species: Pig
• Insert Size (bp): 9300
• Tag / Fusion Protein:
  • Neo EGFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • genbank
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The note that the discrepancies between the full and QC sequence are not of functional concern.