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GOMO-GFP
(Plasmid #60975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60975 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 60975-D50 50 μg of DNA in Tris buffer $495
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DNA 60975-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 2877
  • Total vector size (bp) 11696
  • Vector type
    Plasmodium gene targeting
  • Selectable markers
    hDHFR-yFCU

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    GFP
  • Species
    Synthetic
  • Insert Size (bp)
    726

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    714

Gene/Insert 3

  • Gene/Insert name
    hDHFR-yFCU
  • Species
    Synthetic
  • Insert Size (bp)
    1722

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GOMO-GFP was a gift from Olivier Silvie (Addgene plasmid # 60975 ; http://n2t.net/addgene:60975 ; RRID:Addgene_60975)

  • For your References section:

    A rapid and robust selection procedure for generating drug-selectable marker-free recombinant malaria parasites. Manzoni G, Briquet S, Risco-Castillo V, Gaultier C, Topcu S, Ivanescu ML, Franetich JF, Hoareau-Coudert B, Mazier D, Silvie O. Sci Rep. 2014 Apr 23;4:4760. doi: 10.1038/srep04760. 10.1038/srep04760 PubMed 24755823
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