pCRII HSPB8
(Plasmid
#61035)
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PurposeProbe for HSPB8 for in situ hybridization in zebrafish
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCRII
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHSPB8
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Alt nameheat shock protein b8
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SpeciesD. rerio (zebrafish)
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GenBank IDBC057441
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Entrez Genehspb8 (a.k.a. DKEY-1K23.2, fc09c11, wu:fc04b04, wu:fc09c11, zgc:64202)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
linearize with BamHI, transcribe with T7, probe length is 650.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRII HSPB8 was a gift from Lara Hutson (Addgene plasmid # 61035 ; http://n2t.net/addgene:61035 ; RRID:Addgene_61035) -
For your References section:
Developmental expression patterns of the zebrafish small heat shock proteins. Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD. Dev Dyn. 2008 Feb;237(2):454-63. 10.1002/dvdy.21414 PubMed 18161059