gRNA-hIRF-1 #12
(Plasmid #61079)

• Purpose: Expresses a guide RNA (gRNA) to target human IRF-1 promoter
• Depositing Lab: Hodaka Fujii
• Publication: Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;439(1):132-6. doi: 10.1016/j.bbrc.2013.08.013. Epub 2013 Aug 11.

Backbone

• Vector backbone: pCR-Blunt II-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3400
• Total vector size (bp): 3974
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gRNA_hIRF1 promoter #12
• Species: H. sapiens (human)
• Insert Size (bp): 555
• GenBank ID: DQ789232.1
• Entrez Gene: IRF1 (a.k.a. IMD117, IRF-1, MAR)
• Promoter: U6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13 reverse

Resource Information

• Supplemental Documents:
  • gRNA-hIRF-1 #12.ape
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

gRNA target sequence: CCGGGGGCGCTGGGCTGTCCCGG

For more information on Fujii Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/fujii/