GPKA
(Plasmid
#61091)
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PurposeGFP-tagged cAMP-dependent PKA catalytic subunit
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Depositing Lab
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Depositing OrganizationUniversity of Iowa
Located in United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61091 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 61091-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 61091-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4731
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Modifications to backbonePCR-amplified from RSV-PKA-C-2 (Howard et al. 1991. JBC 266:10189), adding 5’-BspE1 and 3’-XhoI sites, and ligated into EGFP-C1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepkac2
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SpeciesCricetulus griseus
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BspEI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer EGFP-C
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySee publication for cloning details.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Y307H found in Addgene's QC sequence has no known functional consequence.
DNA (Catalog # 61091-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 61091-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GPKA was a gift from Steven Green (Addgene plasmid # 61091 ; http://n2t.net/addgene:61091 ; RRID:Addgene_61091) -
For your References section:
An extranuclear locus of cAMP-dependent protein kinase action is necessary and sufficient for promotion of spiral ganglion neuronal survival by cAMP. Bok J, Zha XM, Cho YS, Green SH. J Neurosci. 2003 Feb 1;23(3):777-87. 23/3/777 [pii] PubMed 12574406
Map uploaded by the depositor.
