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GPKAnes
(Plasmid #61092)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61092 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    EGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Modifications to backbone
    PCR-amplified from RSV-PKA-C-2 (Howard et al. 1991. JBC 266:10189), adding 5’-BspE1 and 3’-XhoI sites, and ligated into EGFP-C1. To make GPKAnes, a double-stranded oligonucleotide encoding the MEK (mitogen-activated protein kinase kinase) nes (QALQKKLEELELDE) (Wu et al., 1992) was added to the C-terminus of the GPKA between HindIII and BamHI.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pkac2
  • Species
    Cricetulus griseus
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspEI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40 poly A Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    See publication for cloning details.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Y307H found in Addgene's QC sequence has no known functional consequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GPKAnes was a gift from Steven Green (Addgene plasmid # 61092 ; http://n2t.net/addgene:61092 ; RRID:Addgene_61092)
  • For your References section:

    An extranuclear locus of cAMP-dependent protein kinase action is necessary and sufficient for promotion of spiral ganglion neuronal survival by cAMP. Bok J, Zha XM, Cho YS, Green SH. J Neurosci. 2003 Feb 1;23(3):777-87. 23/3/777 [pii] PubMed 12574406