pmIL-6 mut AP-1+C/EBP
(Plasmid #61292)

• Purpose: drives luciferase from mouse IL-6 promoter with mutations in both AP-1 & C/EBP sites
• Depositing Lab: Gail Bishop
• Depositing Organization: University of Iowa
  Located in United States of America
• Publication: Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

Backbone

• Vector backbone: pGL2 basic
• Backbone manufacturer: Promega
• Total vector size (bp): 6877
• Modifications to backbone: pGL2-Basic vector was digested with BglII and HindIII. The PCR product generated from primers mIL6BAM and mIL6HIND using CH12.LX genomic DNA as template was digested with BamHI and HindIII, and ligated into the digested pGL2-Basic vector which destroyed the 5' BglII/BamHI cloning site
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: IL-6 promoter
• Species: M. musculus (mouse)
• Mutation: Mutated both AP-1 binding site from TGAGTCT to TGCAGCT and C/EBP binding site from ACATTGTGCAATCT to ACAGATATCAATCT
• Entrez Gene: Il6 (a.k.a. Il-6)
• Promoter: IL-6 promoter with mutant AP-1 and C/EBP binding sites

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: EBV-rev
• 3′ sequencing primer: LucNRev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: See publication for cloning details.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains point mutations in both AP-1 and C/EBP binding sites. See Table 1 of associated publication for more details.

Full sequence and map is available for wild-type vector pmIL-6 FL (Plasmid #61290)

DNA (Catalog # 61292-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 61292-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.