Skip to main content
Addgene
Open Menu

pRB15
(Plasmid #61353)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61353 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pSB1C3
  • Backbone manufacturer
    iGEM
  • Total vector size (bp) 6334
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    indC::synT1 TD
  • Species
    Synthetic; Photorhabdus luminescens and synthetic
  • Mutation
    native IndC T-domain replaced with synthetic T domains
  • Promoter Plac

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Synthetic T domain-encoding DNA sequences were ordered as gBlocks at IDT (Integrated DNA Technologies, Coralville, Iowa).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRB15 was a gift from Barbara Di Ventura & Roland Eils (Addgene plasmid # 61353)

  • For your References section:

    Creating functional engineered variants of the single-module non-ribosomal peptide synthetase IndC by T domain exchange. Beer R, Herbst K, Ignatiadis N, Kats I, Adlung L, Meyer H, Niopek D, Christiansen T, Georgi F, Kurzawa N, Meichsner J, Rabe S, Riedel A, Sachs J, Schessner J, Schmidt F, Walch P, Niopek K, Heinemann T, Eils R, Di Ventura B. Mol Biosyst. 2014 Jul;10(7):1709-18. doi: 10.1039/c3mb70594c. 10.1039/c3mb70594c PubMed 24457530
Related items: