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Purpose4xnrUAS driving GFP
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Depositing Lab
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Depositing OrganizationCarnegie Institution for Science
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61373 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 61373-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 61373-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBT2
- Backbone size w/o insert (bp) 4106
- Total vector size (bp) 5316
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberUnknown
Gene/Insert
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Gene/Insert name4xnr UAS:GFP
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Insert Size (bp)1210
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 20mer
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 61373-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 61373-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4xnrUAS:GFP was a gift from Marnie Halpern (Addgene plasmid # 61373 ; http://n2t.net/addgene:61373 ; RRID:Addgene_61373) -
For your References section:
Transgenerational analysis of transcriptional silencing in zebrafish. Akitake CM, Macurak M, Halpern ME, Goll MG. Dev Biol. 2011 Apr 15;352(2):191-201. doi: 10.1016/j.ydbio.2011.01.002. Epub 2011 Jan 9. 10.1016/j.ydbio.2011.01.002 PubMed 21223961
Map uploaded by the depositor.
