PurposeGateway Middle Entry Vector Containing QS
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61378||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 2555
- Total vector size (bp) 6207
Growth in Bacteria
Insert Size (bp)3652
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 17mer (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Discrepancies between full sequence and Addgene's QC results have no functional consequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pME-QS (L1-L2) was a gift from Marnie Halpern (Addgene plasmid # 61378 ; http://n2t.net/addgene:61378 ; RRID:Addgene_61378)
For your References section:Adoption of the Q transcriptional regulatory system for zebrafish transgenesis. Subedi A, Macurak M, Gee ST, Monge E, Goll MG, Potter CJ, Parsons MJ, Halpern ME. Methods. 2014 Apr 1;66(3):433-40. doi: 10.1016/j.ymeth.2013.06.012. Epub 2013 Jun 20. 10.1016/j.ymeth.2013.06.012 PubMed 23792917