PurposeExpress pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagy
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61459||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepCXN2/ pCAGGS
- Backbone size w/o insert (bp) 7000
- Total vector size (bp) 9000
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth Strain(s)XL10 Gold
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1900
Entrez GeneMAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer GGCTTCTGGCGTGTGACC
- 3′ sequencing primer AGCCACCACCTTCTGATAG (Common Sequencing Primers)
We thank Dr. James Edward Rothman for providing respective pHluorin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEX-PK-hLC3∆G was a gift from Isei Tanida (Addgene plasmid # 61459 ; http://n2t.net/addgene:61459 ; RRID:Addgene_61459)
For your References section:A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy. Tanida I, Ueno T, Uchiyama Y. PLoS One. 2014 Oct 23;9(10):e110600. doi: 10.1371/journal.pone.0110600. eCollection 2014. 10.1371/journal.pone.0110600 PubMed 25340751
Map uploaded by the depositor.