RAB14 3'UTR D2
Purposeluciferase reporter containing RAB14 3' UTR with the second predicted MiR144 binding site deleted
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61491||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepmirGLO Dual-Luciferase
- Backbone size w/o insert (bp) 7350
- Total vector size (bp) 8150
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert nameRAB14 3' UTR
SpeciesH. sapiens (human)
Insert Size (bp)801
Mutationcontains position 837-1637 (numbering relative to start of 3'utr) and second predicted MIR144 binding site deleted, 10bp deletion
Entrez GeneRAB14 (a.k.a. FBP, RAB-14)
- Promoter PGK
/ Fusion Protein
- luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer Luc-F (Common Sequencing Primers)
Oligos used for site-directed mutagenesis:
These 10bp deletion constructs were used in BJH (2015).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RAB14 3'UTR D2 was a gift from Curt Civin (Addgene plasmid # 61491 ; http://n2t.net/addgene:61491 ; RRID:Addgene_61491)
For your References section:MIR144 and MIR451 regulate human erythropoiesis via RAB14. Kim M, Tan YS, Cheng WC, Kingsbury TJ, Heimfeld S, Civin CI. Br J Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164. 10.1111/bjh.13164 PubMed 25312678