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SNAP-EF(N)-20nmSAH-GFP(1-10)
(Plasmid #61661)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61661 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4013
  • Total vector size (bp) 5796
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SNAP-EF(N)-20nmSAH-GFP(1-10)
  • Insert Size (bp)
    1860

Resource Information

Depositor Comments

GFP(1-10) sequence provided by F Pinaud [(2011) PNAS 108:E301-E210] based on work in the lab of G Waldo engineering the split GFP to function as a self assembling bipartite protein [Cabantous et al. (2005) Nat Biotechnol 23:102–107].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SNAP-EF(N)-20nmSAH-GFP(1-10) was a gift from Kristen Verhey (Addgene plasmid # 61661 ; http://n2t.net/addgene:61661 ; RRID:Addgene_61661)
  • For your References section:

    A method for multiprotein assembly in cells reveals independent action of kinesins in complex. Norris SR, Soppina V, Dizaji AS, Schimert KI, Sept D, Cai D, Sivaramakrishnan S, Verhey KJ. J Cell Biol. 2014 Nov 10;207(3):393-406. doi: 10.1083/jcb.201407086. Epub 2014 Nov 3. 10.1083/jcb.201407086 PubMed 25365993