pVenus-N1 cyclin B1 L45A (1324)
(Plasmid
#61851)
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Purposemammalian expression of cyclin B1 L45 A mutant fused to Venus
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61851 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepVenus-N1
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Backbone manufacturerunknown
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecyclin B1
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SpeciesH. sapiens (human)
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MutationL45A
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Entrez GeneCCNB1 (a.k.a. CCNB)
- Promoter CMV
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Tag
/ Fusion Protein
- Venus (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVenus-N1 cyclin B1 L45A (1324) was a gift from Jonathon Pines (Addgene plasmid # 61851 ; http://n2t.net/addgene:61851 ; RRID:Addgene_61851) -
For your References section:
Co-activator independent differences in how the metaphase and anaphase APC/C recognise the same substrate. Matsusaka T, Enquist-Newman M, Morgan DO, Pines J. Biol Open. 2014 Sep 12;3(10):904-12. doi: 10.1242/bio.20149415. 10.1242/bio.20149415 PubMed 25217616