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Purpose(Empty Backbone) UAS transgenesis vector
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62215 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepuc19
- Backbone size (bp) 2644
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Vector typeZebrafish transgenesis
- Promoter UAS-E1b
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Transgenesis vector with tol1 arms, 14xUAS-E1b promoter, ubiquitin C intron, multiple cloning site and ocean pout antifreeze protein terminator. Co-inject with tol1 (not tol2) transposase.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT1UciMP was a gift from Harold Burgess (Addgene plasmid # 62215 ; http://n2t.net/addgene:62215 ; RRID:Addgene_62215) -
For your References section:
Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish. Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, Burgess HA. Nucleic Acids Res. 2015 Jan 27. pii: gkv035. 10.1093/nar/gkv035 PubMed 25628360
Map uploaded by the depositor.
