Purposecontrols the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62244||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8285
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Mutationcatalytically inactive, D10A, H840A
- Promoter Ptet
- Cloning method Unknown
- 5′ sequencing primer kanR
- 3′ sequencing primer rrnB-T1-term-Rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAN-PTet-dCas9 was a gift from Christopher Voigt (Addgene plasmid # 62244 ; http://n2t.net/addgene:62244 ; RRID:Addgene_62244)
For your References section:Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Nielsen AA, Voigt CA. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735. PubMed 25422271