PurposeExpresses gRNA in Leishmania
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63557||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 3740
Vector typeLeishmania Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameguide sequence
- Cloning method Restriction Enzyme
- 5′ cloning site Bbs I (destroyed during cloning)
- 3′ cloning site Bbs I (destroyed during cloning)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe gRNA coding sequence was derived from pX330 plasmid distributed by Addgene ( Dr Feng Zhang, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA).
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The guide sequence will be cloned into the two Bbs I sites (See attached map). This plasmid contains secondary structure and needs to be sequenced with a modified protocol.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSPneoHHgRNAH was a gift from Greg Matlashewski (Addgene plasmid # 63557 ; http://n2t.net/addgene:63557 ; RRID:Addgene_63557)
For your References section:CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani. Zhang WW, Matlashewski G. MBio. 2015 Jul 21;6(4). pii: e00861-15. doi: 10.1128/mBio.00861-15. 10.1128/mBio.00861-15 PubMed 26199327