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(Plasmid #63557)


Item Catalog # Description Quantity Price (USD)
Plasmid 63557 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 3740
  • Vector type
    Leishmania Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    guide sequence
  • gRNA/shRNA sequence
  • Species

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bbs I (destroyed during cloning)
  • 3′ cloning site Bbs I (destroyed during cloning)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gRNA coding sequence was derived from pX330 plasmid distributed by Addgene ( Dr Feng Zhang, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The guide sequence will be cloned into the two Bbs I sites (See attached map). This plasmid contains secondary structure and needs to be sequenced with a modified protocol.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSPneoHHgRNAH was a gift from Greg Matlashewski (Addgene plasmid # 63557 ; ; RRID:Addgene_63557)
  • For your References section:

    CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani. Zhang WW, Matlashewski G. MBio. 2015 Jul 21;6(4). pii: e00861-15. doi: 10.1128/mBio.00861-15. 10.1128/mBio.00861-15 PubMed 26199327