px330-SID4X-dCas9
(Plasmid
#63666)
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Purpose(Empty Backbone) Mammalian gRNA expression vector also expressing SID4X-dCas9
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63666 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepx330
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Backbone manufacturerFeng Zhang
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Vector typeMammalian Expression, CRISPR
- Promoter U6 for gRNA and CMV for dCas9
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer hU6-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
px330-SID4X-dCas9 was a gift from Dong Kong (Addgene plasmid # 63666)