|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63723||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5731
Modifications to backboneInserted translation enhancing element (TEE) and codon optimized iLOV
Vector typeBacterial Expression
- Promoter T7
/ Fusion Protein
- TEE, iLOV, 6xHis, thrombin cleavage site (N terminal on backbone)
Growth in Bacteria
Growth instructionsT7 polymerase required for expression. Gives very high expression levels, consider reduced (0.1 mM) IPTG for induction and/or reduced induction temperature.
Copy numberLow Copy
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28-iLOVf was a gift from Brian Smith (Addgene plasmid # 63723 ; http://n2t.net/addgene:63723 ; RRID:Addgene_63723)