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pET28-iLOVf
(Plasmid #63723)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63723 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size (bp) 5731
  • Modifications to backbone
    Inserted translation enhancing element (TEE) and codon optimized iLOV
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tag / Fusion Protein
    • TEE, iLOV, 6xHis, thrombin cleavage site (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    T7 polymerase required for expression. Gives very high expression levels, consider reduced (0.1 mM) IPTG for induction and/or reduced induction temperature.
  • Copy number
    Low Copy

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Codon optimized iLOV sequence from Andrew Roe & John Christie
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-iLOVf was a gift from Brian Smith (Addgene plasmid # 63723 ; http://n2t.net/addgene:63723 ; RRID:Addgene_63723)