pBAD-HisD-CreiLOV
(Plasmid #192817)

• Purpose: Expresses CreiLOV in bacteria
• Depositing Lab: Kiryl Piatkevich
• Publication: Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:1039317. doi: 10.3389/fbioe.2022.1039317. eCollection 2022.

Backbone

• Vector backbone: pBAD
• Backbone size w/o insert (bp): 3999
• Total vector size (bp): 4359
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Arabose are needed when expressed in bacteria
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CreiLOV
• Species: Chlamydomonas reinhardtii
• Insert Size (bp): 360
• Promoter: araBAD promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GACGCTTTTTATCGCAACTCTCTAC
• 3′ sequencing primer: TTTATCAGACCGCTTCTGCGTTCTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry