PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Twin-Strep tag followed by URA3 selection marker
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63792||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 5000
Vector typeE. coli cloning vector
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameeCFP-Twin-tag followed by URA3
- Promoter none
/ Fusion Protein
- eCFP-Twin-tag (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site BsrGI/Bsp1407I (not destroyed)
- 5′ sequencing primer CTGGCTTAACTATGCGGCATCAG
- 3′ sequencing primer gcaacctgacctacaggaaagag (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The sequence of the eCFP gene has been optimized for S. cerevisiae and generated by gene synthesis. The Twin-Strep tag sequence is adapted from the sequence of commercial Twin-Strep tag plasmids offered by IBA, Goettingen, Germany.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGS1322 was a gift from Monika Golas & Bjoern Sander (Addgene plasmid # 63792 ; http://n2t.net/addgene:63792 ; RRID:Addgene_63792)
For your References section:Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by Homologous Recombination and Characterization of Endogenous Macromolecular Assemblies in Saccharomyces cerevisiae. Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard JR, Golas MM, Sander B. Mol Biotechnol. 2014 Jun 27. 10.1007/s12033-014-9778-5 PubMed 24969434