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pAraH6HATT
(Plasmid #63901)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63901 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAra
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TT Fab
  • Alt name
    human Fab to tetanus toxin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • Promoter araBAD
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • HA tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer 5'-AAG ACA GCT ATC GCG ATT GCA G-3'
  • 3′ sequencing primer 5'-AGA AGC GTA GTC CGG AAC GTC-3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Provided with the pComb3H system for arabinose induced expression of soluble protein with detection tags. The “TT” refers to the human Fab to tetanus toxin which can be used as a protein expression control. Alternatively, one could upgrade to the pComb3X system, also available on Addgene, which already contains tags.

Select reference:

Cary, S.P.; Lee, J.; Wagenknecht, R.; Silverman, G.J. (2000) Characterization of Superantigen-Induced Clonal Deletion with a Novel Clan III-Restricted Avian Monoclonal Antibody: Exploiting Evolutionary Distance to Create Antibodies Specific for a Conserved VH Region Surface. J. Immun., 164, 4730-4741.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAraH6HATT was a gift from Carlos Barbas (Addgene plasmid # 63901 ; http://n2t.net/addgene:63901 ; RRID:Addgene_63901)
  • For your References section:

    Synthetic Human Antibodies: Selecting and Evolving Functional Proteins Methods, A companion to Methods in Enzymology 8, 94-103. Barbas CF, Wagner J. Methods, A companion to Methods in Enzymology, 1995; 8(2): 94-103. 10.1006/meth.1995.9997