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pComb3HTT
(Plasmid #63900)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63900 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pComb3H
  • Backbone size w/o insert (bp) 3300
  • Total vector size (bp) 4700
  • Vector type
    Bacterial Expression ; phage display

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TT Fab
  • Alt name
    human Fab to tetanus toxin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • Promoter lacZ
  • Tag / Fusion Protein
    • geneIII (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer 5'-AAG ACA GCT ATC GCG ATT GCA G-3'
  • 3′ sequencing primer 5'-GCC CCC TTA TTA GCG TTT GCC ATC-3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

pComb3H is the second generation of pComb vector. Improvements over pComb3 include increased stability and introduction of a new cloning region utilizing asymmetrical SfiI restriction sites for directional cloning of full Fab, scFv, peptide and other proteins for phage display. The “TT” refers to the human Fab to tetanus toxin which can be used as a phage display and protein expression control. Soluble Fab can be expressed by removing the gene for the pIII phage fusion protein by SpeI/NheI digest. Sufficient soluble protein is also found in the periplasmic space as a result of proteolysis.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pComb3HTT was a gift from Carlos Barbas (Addgene plasmid # 63900)