PurposeBackbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63900||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3300
- Total vector size (bp) 4700
Vector typeBacterial Expression ; phage display
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
Gene/Insert nameTT Fab
Alt namehuman Fab to tetanus toxin
SpeciesH. sapiens (human)
Insert Size (bp)1400
- Promoter lacZ
/ Fusion Protein
- geneIII (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SfiI (not destroyed)
- 3′ cloning site SfiI (not destroyed)
- 5′ sequencing primer 5'-AAG ACA GCT ATC GCG ATT GCA G-3'
- 3′ sequencing primer 5'-GCC CCC TTA TTA GCG TTT GCC ATC-3' (Common Sequencing Primers)
Terms and Licenses
pComb3H is the second generation of pComb vector. Improvements over pComb3 include increased stability and introduction of a new cloning region utilizing asymmetrical SfiI restriction sites for directional cloning of full Fab, scFv, peptide and other proteins for phage display. The “TT” refers to the human Fab to tetanus toxin which can be used as a phage display and protein expression control. Soluble Fab can be expressed by removing the gene for the pIII phage fusion protein by SpeI/NheI digest. Sufficient soluble protein is also found in the periplasmic space as a result of proteolysis.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pComb3HTT was a gift from Carlos Barbas (Addgene plasmid # 63900)