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(Plasmid #64146)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 64146 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pCold TF
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5769
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    Pleckstrin homology domain-containing family M member 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PLEKHM1 (a.k.a. AP162, B2, OPTB6)
  • Promoter cspA
  • Tags / Fusion Proteins
    • 6xHis-tag (N terminal on insert)
    • Trigger Factor (TF) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Kazusa DNA Research Institute (Kisarazu, Japan)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCold-TF-hPLEKHM1 was a gift from Tamotsu Yoshimori (Addgene plasmid # 64146 ; ; RRID:Addgene_64146)
  • For your References section:

    Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain. Tabata K, Matsunaga K, Sakane A, Sasaki T, Noda T, Yoshimori T. Mol Biol Cell. 2010 Dec;21(23):4162-72. doi: 10.1091/mbc.E10-06-0495. Epub 2010 Oct 13. 10.1091/mbc.E10-06-0495 PubMed 20943950