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pHLUM (version 2)
(Plasmid #64166)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64166 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS313
  • Modifications to backbone
    Deleted XhoI and BamHI restriction sites from multiple cloning site by modification of codons, preserving the amino acid sequence of lacZ Introduced an AatII restriction site 5' and XhoI and BamHI sites 3' of HIS3 marker gene Cloned fragment containing LEU2, URA3 and MET17 from pHLUM to XhoI/BamHI RS.
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2, URA3, HIS3 ; MET17

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHLUM (version 2) was a gift from Markus Ralser (Addgene plasmid # 64166 ; http://n2t.net/addgene:64166 ; RRID:Addgene_64166)