human PNP non-tagged
(Plasmid
#64199)
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PurposeExpression of native human PNP (non-tagged)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64199 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCR-T7/CT-TOPO
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Backbone manufacturerInvitrogen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePurine Nucleoside Phosphorylase
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Alt namePNP
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SpeciesH. sapiens (human)
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Entrez GenePNP (a.k.a. NP, PRO1837, PUNP)
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Tag
/ Fusion Protein
- none
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
human PNP non-tagged was a gift from Vern Schramm (Addgene plasmid # 64199 ; http://n2t.net/addgene:64199 ; RRID:Addgene_64199) -
For your References section:
Neighboring group participation in the transition state of human purine nucleoside phosphorylase. Murkin AS, Birck MR, Rinaldo-Matthis A, Shi W, Taylor EA, Almo SC, Schramm VL. Biochemistry. 2007 May 1;46(17):5038-49. Epub 2007 Apr 4. 10.1021/bi700147b PubMed 17407325
