PurposeBicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64205||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7752
- Total vector size (bp) 9582
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameBicaudal D protein
SpeciesM. musculus (mouse)
Insert Size (bp)1830
Mutationno start methionine, aa1-594
- Promoter chicken Beta Actin
/ Fusion Proteins
- tdTomato (N terminal on backbone)
- flag (N terminal on insert)
- FKBP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BsrGI (not destroyed)
- 3′ cloning site RsrII (not destroyed)
- 5′ sequencing primer TTCGGCTTCTGGCGTGTGAC
- 3′ sequencing primer GATGAGTTTGGACAAACCAC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bybicaudal D2 mouse from ATCC
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBa-tdTomato-flag-BicD2 594-FKBP was a gift from Gary Banker & Marvin Bentley (Addgene plasmid # 64205 ; http://n2t.net/addgene:64205 ; RRID:Addgene_64205)
For your References section:A novel assay reveals preferential binding between Rabs, kinesins, and specific endosomal subpopulations. Bentley M, Decker H, Luisi J, Banker G. J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26. 10.1083/jcb.201408056 PubMed 25624392