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pGH00.0126
(Plasmid #64257)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64257 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBin19 transformation vector
  • Backbone manufacturer
    Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 12:8711–8721
  • Total vector size (bp) 15608
  • Vector type
    Plant Expression ; Binary Agrobacterium vector for plant transformation.

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    EGFP
  • Promoter E12-omega

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    neomycin phosphotransferase II
  • Alt name
    NPTII
  • Promoter E12-omega

Cloning Information for Gene/Insert 2

Resource Information

Depositor Comments

E12-omega is a strong constitutive promoter obtained from pE2113-GUS (Mitsuhara et al. 1996). The promoter consists of two directly repeated copies the 5’ upstream sequence of the cauliflower mosaic virus (CaMV) 35S promoter (-419 to -90), followed by one copy of the P35S, the 5’-upstream sequence of the CaMV 35S promoter (–90 to –1) and the 5’ Omega-untranslated sequence of the tobacco mosaic virus. The Omega sequence is known to enhance translation levels in plant, animal and E. coli. This chimeric promoter was shown to support very high protein expression levels (approx. 23-fold higher then the standard CaMV 35S promoter) based on high transcription and enhanced translation rates (Mitsuhara et al. 1996).

Note-there is a 2nd NPTII cassette under the NOS Promoter. Only 1 NPTII region mutation was corrected and that is noted in the GenBank record.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGH00.0126 was a gift from Mark Guiltinan & Siela Maximova (Addgene plasmid # 64257 ; http://n2t.net/addgene:64257 ; RRID:Addgene_64257)
  • For your References section:

    Stable transformation of Theobroma cacao L. and influence of matrix attachment regions on GFP expression. Maximova S, Miller C, Antunez de Mayolo G, Pishak S, Young A, Guiltinan MJ. Plant Cell Rep. 2003 Jun;21(9):872-83. Epub 2003 Apr 3. 10.1007/s00299-003-0596-7 PubMed 12789505