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GFP-PCAF (delta5-53a.a.)
(Plasmid #65388)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65388 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 65388-D50 50 μg of DNA in Tris buffer $495
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DNA 65388-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA6.2/N-EmGFP-DEST
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5847
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCAF
  • Alt name
    KAT2B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2349
  • Mutation
    lost 13-159bp of the ORF
  • Entrez Gene
    KAT2B (a.k.a. CAF, P/CAF, PCAF)
  • Promoter CMV
  • Tags / Fusion Proteins
    • EmGFP (N terminal on backbone)
    • V5 (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer EGFP_C_primer
  • 3′ sequencing primer TK polyA reverse primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene Source: Harvard PlasmID HsCD00343111; lost 5-53a.a. during the PCR process

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-PCAF (delta5-53a.a.) was a gift from Kyle Miller (Addgene plasmid # 65388 ; http://n2t.net/addgene:65388 ; RRID:Addgene_65388)

  • For your References section:

    Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M, Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM. Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114. 10.1101/gad.252189.114 PubMed 25593309
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