pTGFP-T7-Hha10T
(Plasmid #65427)

• Purpose: (Empty Backbone) Parent vector for constructing non-replicating lesion-bearing plasmid
• Depositing Lab: Yinsheng Wang
• Publication: You et al Nat Protoc. 2015 Sep;10(9):1389-406. doi: 10.1038/nprot.2015.094. Epub 2015 Aug 20.

Backbone

• Vector backbone: pTurboGFPN
• Backbone manufacturer: EvroGen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Prof. Timothy R. O'Connor (City of Hope) provided the original vector, i.e., pTGFP-Hha10, which were modified from pTurboGFPN plasmid (EvroGen, San Diego) . (Pubmed ID: 17507378).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pTGFP-Hha10 vector was used to generate the parent vector, i.e., pTGFP-T7-Hha10T. This parent vector can be used to construct non-replicating double-stranded plasmids that contain a site-specific DNA lesion.

Note: Addgene's quality control sequencing finds a T18N amino acid residue substitution in TurboGFP. This residue change is not thought to affect plasmid function for transcriptional mutagenesis assay.