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pLPCX-Cx43-FS-IRES-GFP
(Plasmid #65438)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65438 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLPCX
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6264
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cx43
  • Alt name
    Connexin 43
  • Alt name
    GJA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2575
  • Mutation
    frameshift (FS) mutation 35delA
  • GenBank ID
    2697
  • Entrez Gene
    GJA1 (a.k.a. AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA)
  • Promoter CMV
  • Tag / Fusion Protein
    • IRES-GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer LNCX-3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Initially cloned into pBluescript: Cx43 insert digested out with EcoRV from Addgene plasmid #27383 and IRES-GFP digested from pIRES2-EGFP Addgene plasmid 3178 from clontech using BamHI-NotI. Entire Cx43-IRES-GFP cloned from pBluescript into pLPCX using XhoI and NotI.
  • Terms and Licenses

Depositor Comments

FrameShift Mutation at EcoNI site of Cx43 (35delA) leading to out of frame synthesis after 11 amino acid and premature stop at amino acid 100.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPCX-Cx43-FS-IRES-GFP was a gift from Trond Aasen (Addgene plasmid # 65438 ; http://n2t.net/addgene:65438 ; RRID:Addgene_65438)
  • For your References section:

    Internal translation of the connexin 43 transcript. Salat-Canela C, Sese M, Peula C, Ramon y Cajal S, Aasen T. Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31. 10.1186/1478-811X-12-31 PubMed 24884945