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pIRESpuro2 AXL
(Plasmid #65627)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65627 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIRESpuro2
  • Backbone manufacturer
    CloneTech
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 7805
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tyrosine-protein kinase receptor UFO
  • Alt name
    AXL
  • Alt name
    UFO
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2838
  • Mutation
    Spurious PDPRPHR on C terminus
  • GenBank ID
    NM_001699.5
  • Entrez Gene
    AXL (a.k.a. ARK, AXL3, JTK11, Tyro7, UFO)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRESpuro2 AXL was a gift from Aaron Meyer (Addgene plasmid # 65627 ; http://n2t.net/addgene:65627 ; RRID:Addgene_65627)
  • For your References section:

    The AXL Receptor is a Sensor of Ligand Spatial Heterogeneity. Meyer AS, Zweemer AJ, Lauffenburger DA. Cell Syst. 2015 Jul 29;1(1):25-36. 10.1016/j.cels.2015.06.002 PubMed 26236777