pC239
(Plasmid #65953)

• Purpose: N2 inverter plasmid for two-strain system
• Depositing Lab: Matthew Bennett
• Publication: Chen et al Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.

Backbone

• Vector backbone: p15A
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: AiiA
• Species: Bacillus thuringiensis
• Insert Size (bp): 786
• Promoter: pCin*
• Tag / Fusion Protein:
  • LAA tagged (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: gagaaaactagtatgacagtaaagaaactttatttcatcccagcag
• 3′ sequencing primer: ggaccagagctcttattacgctgcaagggcgtaattttcgtcgttcgctgc

Gene/Insert 2

• Gene/Insert name: LacI
• Insert Size (bp): 1122
• Promoter: pCin
• Tag / Fusion Protein:
  • LAA tagged (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: gagaaaactagtatgaaaccagtaacgttatacgatgtcgcag
• 3′ sequencing primer: taggggaagcttttattacgctgcaagggcgtaattttcgtcgttcgctgc

Resource Information

• Supplemental Documents:
  • annotated file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry