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pEGIP-Cas9Sp-target
(Pooled library #66120)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Pooled Library 66120 Back-ordered
 Shipped as suspended DNA in a microcentrifuge tube 		1 $

Backbone

  • Vector backbone
    pEGIP
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Synthesized target site library for Cas9 S. pyogenes covering ~1400 sites
  • Species
    Synthetic

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGIP-Cas9Sp-target was a gift from George Church (Addgene plasmid # 66120)

  • For your References section:

    Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Chari R, Mali P, Moosburner M, Church GM. Nat Methods. 2015 Sep;12(9):823-6. doi: 10.1038/nmeth.3473. Epub 2015 Jul 13. 10.1038/nmeth.3473 PubMed 26167643
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