pEGIP-Cas9St1-target
(Pooled library
#66121)
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PurposeTarget site library corresponding to the ~1400 sgRNAs in pLKO-Cas9st1
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Pooled Library | 66121 |
Back-ordered Shipped as suspended DNA in a microcentrifuge tube |
1 | $ | |
Backbone
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Vector backbonepEGIP
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Vector typeMammalian Expression, Lentiviral, CRISPR, Synthetic Biology
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSynthesized target site library for Cas9 S. thermophilus covering ~1400 sites
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GGATGCATGATCTAGGGCCTCGTCT
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGIP-Cas9St1-target was a gift from George Church (Addgene plasmid # 66121) -
For your References section:
Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach. Chari R, Mali P, Moosburner M, Church GM. Nat Methods. 2015 Sep;12(9):823-6. doi: 10.1038/nmeth.3473. Epub 2015 Jul 13. 10.1038/nmeth.3473 PubMed 26167643