NmCitrine-PHdomain
(Plasmid
#66783)
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PurposeExpression of PIP2 binding protein in sea urchin embryos
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Depositing Lab
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Depositing OrganizationUniversity of California, San Diego (UCSD)
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66783 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 66783-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 66783-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2+8
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Backbone manufacturerAddgene Plasmid #3493, Gökirmak et al. 2012, JBC
- Backbone size w/o insert (bp) 4851
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Vector typeMammalian Expression ; sea urchin, zebrafish, xenopus
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePLCdelta
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Alt namePLCD1
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SpeciesH. sapiens (human)
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Insert Size (bp)529
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Mutationonly contains PH domain
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Entrez GenePLCD1 (a.k.a. NDNC3, PLC-III)
- Promoter SP6
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Tag
/ Fusion Protein
- mCitrine (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site FseI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer TGTTGTTAACTTGTTTATTGCAGCT
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene plasmid 21179 was used for PH domain sequence
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 66783-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 66783-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NmCitrine-PHdomain was a gift from Amro Hamdoun (Addgene plasmid # 66783 ; http://n2t.net/addgene:66783 ; RRID:Addgene_66783) -
For your References section:
Migration of sea urchin primordial germ cells. Campanale JP, Gokirmak T, Espinoza JA, Oulhen N, Wessel GM, Hamdoun A. Dev Dyn. 2014 Jul;243(7):917-27. doi: 10.1002/dvdy.24133. Epub 2014 Apr 30. 10.1002/dvdy.24133 PubMed 24677545