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CmCitrine Lck w/ NosUTRs
(Plasmid #66785)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 66785 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3000
  • Vector type
    sea urchin, zebrafish, xenopus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    LCK proto-oncogene, Src family tyrosine kinase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Tandem repeat of LCK the N-terminal membrane targeting sequence: MGCIKSKRKDNLNDDEAAMGCIKSKRKDNLNDDEAPVAT
  • Entrez Gene
    LCK (a.k.a. IMD22, LSK, YT16, p56lck, pp58lck)
  • Promoter T7
  • Tags / Fusion Proteins
    • 5' UTR from sea urchin Nanos (N terminal on insert)
    • mCitrine (C terminal on insert)
    • 3' UTR from sea urchin Nanos (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ApaI (not destroyed)
  • 3′ cloning site NdeI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Lck was taken from Addgene plasmid #53749

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The membrane anchoring of mCitrine in sea urchin germ cells uses the sea urchin (S. purpuratus) 5' and 3' UTRs Nanos2 UTRs flanking this insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CmCitrine Lck w/ NosUTRs was a gift from Amro Hamdoun (Addgene plasmid # 66785 ; ; RRID:Addgene_66785)
  • For your References section:

    Migration of sea urchin primordial germ cells. Campanale JP, Gokirmak T, Espinoza JA, Oulhen N, Wessel GM, Hamdoun A. Dev Dyn. 2014 Jul;243(7):917-27. doi: 10.1002/dvdy.24133. Epub 2014 Apr 30. 10.1002/dvdy.24133 PubMed 24677545