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(Plasmid #36871)


Item Catalog # Description Quantity Price (USD)
Plasmid 36871 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 5466
  • Vector type
    Mammalian Expression, Mouse Targeting ; xenopus/mammalian/avian/zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Src homology-2 domain (dSH2)
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Src (a.k.a. pp60c-src)
  • Promoter CMV
  • Tags / Fusion Proteins
    • myc (N terminal on insert)
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor notes that the discrepancies between the full sequence and the Addgene QC sequences do not affect the function of this sensor.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-myc-gfp-dSH2 was a gift from Timothy Gomez (Addgene plasmid # 36871 ; ; RRID:Addgene_36871)
  • For your References section:

    Src-dependent tyrosine phosphorylation at the tips of growth cone filopodia promotes extension. Robles E, Woo S, Gomez TM. J Neurosci. 2005 Aug 17;25(33):7669-81. 10.1523/JNEUROSCI.2680-05.2005 PubMed 16107653