pLL3.7 EGFPC2 TAZ
(Plasmid #66850)

• Purpose: Express GFP-fused TAZ by lentivirus
• Depositing Lab: Yutaka Hata
• Publication: Bao et al J Biochem. 2011 Aug;150(2):199-208. doi: 10.1093/jb/mvr063. Epub 2011 May 17.

Backbone

• Vector backbone: pLL3.7
• Backbone manufacturer: Addgene
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: WWTR1/TAZ
• Species: H. sapiens (human)
• Entrez Gene: WWTR1 (a.k.a. TAZ)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: T3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

I isolated NheI/MfeI fragment from pClneoEGFPC2 TAZ and subcloned into NheI/EcoRi sites of the original pLL3.7. That is the reason why T3 promtoer can be used for the sequencing. That sequence is derived from pClneo.

Please note: Plasmid is missing 444bp compared to pLL3.7 backbone sequence. This removes the KS primer and loxP at 5' end of the insert, but is not expected to affect plasmid function.