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PurposeExpress GFP-fused TAZ by lentivirus
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Depositing Lab
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Depositing OrganizationInstitute of Science Tokyo
Located in Japan -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66850 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 66850-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 66850-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepLL3.7
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Backbone manufacturerAddgene
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameWWTR1/TAZ
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SpeciesH. sapiens (human)
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Entrez GeneWWTR1 (a.k.a. TAZ)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer T3
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
I isolated NheI/MfeI fragment from pClneoEGFPC2 TAZ and subcloned into NheI/EcoRi sites of the original pLL3.7. That is the reason why T3 promtoer can be used for the sequencing. That sequence is derived from pClneo.
Please note: Plasmid is missing 444bp compared to pLL3.7 backbone sequence. This removes the KS primer and loxP at 5' end of the insert, but is not expected to affect plasmid function.
DNA (Catalog # 66850-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 66850-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLL3.7 EGFPC2 TAZ was a gift from Yutaka Hata (Addgene plasmid # 66850 ; http://n2t.net/addgene:66850 ; RRID:Addgene_66850) -
For your References section:
A cell-based assay to screen stimulators of the Hippo pathway reveals the inhibitory effect of dobutamine on the YAP-dependent gene transcription. Bao Y, Nakagawa K, Yang Z, Ikeda M, Withanage K, Ishigami-Yuasa M, Okuno Y, Hata S, Nishina H, Hata Y. J Biochem. 2011 Aug;150(2):199-208. doi: 10.1093/jb/mvr063. Epub 2011 May 17. 10.1093/jb/mvr063 PubMed 21586534
