pET30Xa-SBL1(160S)
(Plasmid #66872)

• Purpose: Expresses WTsoybean lipoxygenase-1 160S with N-term Histag
• Depositing Lab: Betty Gaffney
• Publication: Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-66. Epub 2005 Sep 12.

Backbone

• Vector backbone: pET30Xa-LIC
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5444
• Total vector size (bp): 7978
• Modifications to backbone: 11 bp C-terminal extension: TAAACTAGTCATGGGGC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: 37C before induction, 30 deg for 14-16+ hrs after induction
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: soybean lipoxygenase-1 wild type
• Alt name: SBL1
• Species: Glycine max
• Mutation: none
• GenBank ID: AAA33986.1
• Entrez Gene: NEWENTRY
• Promoter: T7lac
• Tag / Fusion Protein:
  • Histag from pET30Xa-LIC (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: tca tca ttc ttc tgg tct gg
• 3′ sequencing primer: ttg ccg act ctc att agc c

Resource Information

• Supplemental Documents:
  • B.C. Maguire, F. Wu, B. Washburn and B.J. Gaffney, unpublished.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

prepared from pET30Xa-SBL1(160E) by site directed mutagenesis