PDX1-2A-eGFP
(Plasmid #66964)

• Purpose: PDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus
• Depositing Lab: Danwei Huangfu
• Publication: Zhu et al Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 10.1016/j.stemcr.2015.04.016.

Backbone

• Vector backbone: pBlueScript SKII (+)
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 4969
• Vector type: Unspecified

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pancreatic and duodenal homeobox 1
• Alt name: PDX1 homology arms
• Species: H. sapiens (human)
• GenBank ID:
• Entrez Gene: PDX1 (a.k.a. GSF, IDX-1, IPF1, IUF1, MODY4, PAGEN1, PDX-1, STF-1)
• Tag / Fusion Protein:
  • 2A-eGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: xbaI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry