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(Plasmid #66986)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66986 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
    OCT4-2A-eGFP-PGKPuro plasmid (Addgene: 31938)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5424
  • Total vector size (bp) 6135

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (unknown if destroyed)
  • 3′ cloning site Asc1 (unknown if destroyed)
  • 5′ sequencing primer CTCACTTCACTGCACTGTACTCCT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OCT4-2A-mOrange was a gift from Danwei Huangfu (Addgene plasmid # 66986 ; ; RRID:Addgene_66986)
  • For your References section:

    A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells. Zhu Z, Verma N, Gonzalez F, Shi ZD, Huangfu D. Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 10.1016/j.stemcr.2015.04.016. 10.1016/j.stemcr.2015.04.016 PubMed 26028531