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A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells.
Zhu Z, Verma N, Gonzalez F, Shi ZD, Huangfu D
Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 10.1016/j.stemcr.2015.04.016.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
66964PDX1-2A-eGFPPDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus
66986OCT4-2A-mOrangeGenerate OCT4 mOrange reporter line without selection cassette
66989Oct4-Cr1Expresses gRNA that targets stop codon for OCT4