A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells.

Zhu Z, Verma N, Gonzalez F, Shi ZD, Huangfu D
Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 10.1016/j.stemcr.2015.04.016. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
66964	PDX1-2A-eGFP	PDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus
66986	OCT4-2A-mOrange	Generate OCT4 mOrange reporter line without selection cassette
66989	Oct4-Cr1	Expresses gRNA that targets stop codon for OCT4