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pUMV19
(Plasmid #67161)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67161 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC118
  • Backbone size w/o insert (bp) 3200
  • Total vector size (bp) 9900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    the mevalonate pathway genes
  • Alt name
    HMGS, HMGR, IDI, MVK, PMVK, DPMVD
  • Species
    Streptomyces sp. CL190
  • Insert Size (bp)
    6792
  • GenBank ID
    AB037666
  • Promoter lacZ

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer M13 Rev
  • 3′ sequencing primer M13 F20
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Takahashi S et al J Bacteriol 1999 Feb;181(4):1256-63.
Kaneda K et al Proc Natl Acad Sci USA 2001 Jan 30;98(3):932-7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUMV19 was a gift from Tomohisa Kuzuyama (Addgene plasmid # 67161 ; http://n2t.net/addgene:67161 ; RRID:Addgene_67161)
  • For your References section:

    A gene cluster for the mevalonate pathway from Streptomyces sp. Strain CL190. Takagi M, Kuzuyama T, Takahashi S, Seto H. J Bacteriol. 2000 Aug;182(15):4153-7. PubMed 10894721