PurposeExpression of hArl8b with C-term V5 and His6 tag in E. coli
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67425||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerLife Technologies
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneARL8B (a.k.a. ARL10C, Gie1)
- Promoter T7/lacO
/ Fusion Proteins
- V5 (C terminal on backbone)
- His (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-DEST42-ARL8B-V5-His6 was a gift from Richard Kahn (Addgene plasmid # 67425 ; http://n2t.net/addgene:67425 ; RRID:Addgene_67425)