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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWIPI3
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SpeciesH. sapiens (human)
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Insert Size (bp)1035
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Mutationgene sequence is codon optimized for expression in E. coli
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Entrez GeneWDR45B (a.k.a. NEDSBAS, WDR45L, WIPI-3, WIPI3)
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Tag
/ Fusion Protein
- GST-TEV (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-WIPI3 was a gift from James Hurley (Addgene plasmid # 42527 ; http://n2t.net/addgene:42527 ; RRID:Addgene_42527) -
For your References section:
Two-site recognition of phosphatidylinositol 3-phosphate by PROPPINs in autophagy. Baskaran S, Ragusa MJ, Boura E, Hurley JH. Mol Cell. 2012 Aug 10;47(3):339-48. doi: 10.1016/j.molcel.2012.05.027. Epub 2012 Jun 14. 10.1016/j.molcel.2012.05.027 PubMed 22704557