pGEX-4T-1-Shp2 SH2s
(Plasmid
#67575)
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PurposeExpresses GST-tagged Shp2 SH2 domains in bacteria
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67575 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX-4T-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSrc homology 2 domain-containing phosphatase 2 SH2s
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Alt nameShp2
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SpeciesM. musculus (mouse)
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MutationDeleted amino acids 219-597
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T-1-Shp2 SH2s was a gift from Graeme Carnegie (Addgene plasmid # 67575 ; http://n2t.net/addgene:67575 ; RRID:Addgene_67575) -
For your References section:
Protein Kinase A (PKA) Phosphorylation of Shp2 Protein Inhibits Its Phosphatase Activity and Modulates Ligand Specificity. Burmeister BT, Wang L, Gold MG, Skidgel RA, O'Bryan JP, Carnegie GK. J Biol Chem. 2015 May 8;290(19):12058-67. doi: 10.1074/jbc.M115.642983. Epub 2015 Mar 23. 10.1074/jbc.M115.642983 PubMed 25802336